This DNA library is ideally suited for downstream analysis of both SNPs and CNV in single cells, due to its low rate of preferential amplification.1™ WGA Kit is designed to provide optimal whole genome amplification—not only from samples containing DNA from a few cells, but from DNA obtained from one single cell.
applied prepared samples for the ratiometric array, and L.
WGA protocol is a well-balanced library of approximately 19 million fragments representing the entire genome.
This has not been validated at Rubicon Genomics, but some customers have successfully used PVA (Polyvinyl Alcohol; e.g. Additionally, your washing/collecting buffer (PBS) must be also Ca- and Mg-free. Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. What is the sample input volume for Pico PLEX WGA Kit? Does Rubicon Genomics have an NGS-ready kit for single cell applications? What is the expected genomic locus representation and Loss of Heterozygosity (LOH) for Pico PLEX WGA products?
This kit will accommodate a 5 μl sample input volume per reaction (with a maximum of 2.5 μl of cell & buffer carryover). Can the product of Pico PLEX WGA Kit be taken straight to NGS? Yes, Pico PLEX DNA-seq can be used with Illumina NGS platforms. Sigma-Aldrich sells a Genome Plex WGA kit, is it the same technology as Pico PLEX WGA? For reference purposes, see the data obtained from a third party in the table below.
(a–c) The data in each column are from a single lectin: WGA (a), HPA (b) and AOL (c).
The top row shows representative examples of lectin histochemistry for WGA-555 (yellow) (a, top), HPA-488 (green) (b, top) and AOL-555 (yellow) (c, top); a DAPI counterstain highlights the cell nuclei in blue (magnification, ×20).
The sample containing 0 cells (green lines; no template control) shows very low background.
Single-blastomere biopsies were amplified using Pico PLEX WGA Kit, labeled and hybridized to Blue Gnome’s 24Sure arrays at Genesis Genetics Institute. In 2011, ESHRE clinical trials confirmed accuracy of karyotyping using Pico PLEX technology.
The second row of images shows the corresponding sequential section stained with H&E for each lectin. staining for each lectin, with the n number of samples shown below each bar in the graphs.
The top row of graphs shows the staining for the apical epithelial membrane (the part of the cell membrane that lines the esophageal lumen). performed the experimental work and data analysis with help from A.
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